Fig. 1. Developmental dynamics of glial cells in normal embryonic PNS. (A-C) Double labeling using a glial marker REPO (black) and a neuronal marker ELAV (orange) in the PNS (single abdominal segment; dorsal up, anterior to left). Three types of REPO-positive glial cells are present in an abdominal hemisegment of the embryonic PNS; one dbd support glial cell (DBDG), one PG3 cell (arrowhead) and five ligament cells of the lateral chordotonal organ (black asterisk). (A) Stage 12 embryo. (B) Stage 14 embryo. (C) Stage 16 embryo. The dbd neuron (white asterisk) can be identified at the dorsal side of the DBDG in late stage embryos. d, dorsal cluster of sensory neurons. (D) Summary of developmental dynamics of the REPO-positive glial cells (black) (single abdominal segment; dorsal up, anterior to left). PG3 undergoes extensive ventral migration between stage 12 and stage 16. The dorsoventral positions of the PG3 and DBDG are reversed between these two stages. lig., ligament cells.
