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Fig. 5. Roles of the Notch signaling pathway in the dbd and dda lineages. (A,B) The dbd lineage in stage 16 embryos (single abdominal segment; dorsal up, anterior to left). The dorsal cluster of sensory neurons are stained brown (cytoplasm) with mAb 22C10. The dbd neuron is indicated by an arrowhead. DBDG (arrow) is stained black with anti-REPO (nucleus). (A) Normal embryo. (B) Ectopic REPO expression is observed in the nucleus of the dbd neuron when a constitutively active form of Notch is expressed in neurons (UAS-Notchact/C155-GAL4). Note that the morphology of the neuron is also transformed towards that of a glial cell. (C,D) Double labeling of REPO expression (black) and ELAV expression (orange) in the dorsal cluster of PNS. (C) Stage 13 normal embryo. (D) Stage 13 numb1 embryo. The dda neuron (white arrowhead) and the dbd neuron (black arrowhead) ectopically express REPO. ELAV expression is still observed in the cytoplasm of the dda neuron as well as in the dbd neuron, but not in DBDG (arrow). (E-H) Nubbin expression in the dorsal cluster of sensory neurons at stage 16. In normal embryos (E), Nubbin expression is restricted to the dbd neuron (black arrowhead) and the dda neuron (white arrowhead). Both neurons are absent in C155-GAL4/UAS-Notchact embryos (F), and in numb1 embryos (G). Both neurons are duplicated in sanpodoC55 embryos (H). (I-K) gcm mRNA expression in the dbd lineage at stage 12 (single abdominal segment. dorsal up, anterior to left). (I) gcm expression is observed in the presumptive DBDG (arrow) of normal embryos. (J) C155-GAL4/UAS-Notchact gain-of-function embryo. Ectopic gcm expression is observed in the presumptive dbd neuron (arrowhead). (K) numb1 mutant embryo. Ectopic gcm expression is observed in the presumptive dbd neuron (arrowhead). In contrast, gcm expression is absent in the presumptive PG3 and the ligament cells. (L) C155-GAL4/UAS- Notchact embryo at stage 16 (single abdominal segment; dorsal up, anterior to left). Ectopic gcm expression is maintained in the dbd neuron that is transformed into a glial cell (black arrowhead). gcm expression in endogenous DBDG has already disappeared by this stage (arrow). An extra gcm-positive cell (open arrowhead) is also observed in the dorsal cluster of sensory neurons.





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