Fig. 4. Tsh and Hth expression in genetic mosaics. (A) vg^83b27r mutant clones were induced between 48 and 72 hours after egg laying. Early third instar larvae were examined 2 days after clone induction, so that the majority of clones examined were induced in early second instar. Clones were labeled by absence of ß-gal (blue). Hth (red) was ectopically expressed in the clone. Tsh (green) was not. Higher magnification views of the boxed region are shown separately for each channel at right. 11 of 13 clones examined expressed ectopic Hth, but Not Tsh. The relatively large size of the clone shown here may reflect some growth prior to wing pouch specification. (B) Clones expressing Vestigial (Act>Gal4 UAS-Vg UAS-GFP) induced during second instar and examined in mature third instar discs. Vestigial-expressing clones marked by co-expression of GFP (blue). Hth (red) levels were reduced in the mutant cells. Tsh (green) levels were unaffected relative to adjacent wild-type cells. Note that the Vg-expressing clones tend to sort out from the disc (arrowhead) and so the nuclei of the cells are not uniformly in the same focal plane. (C) nub¹ mutant clones induced in early third instar 72-96 hours after egg laying. Clones were labeled by absence of ß-gal. Hth was ectopically expressed in a nub¹ clone whereas Tsh was not (arrow).

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