Fig. 3. Wild-type function of zpg is required for survival of differentiating female germ cells. (A,B) Ovarioles and (C-F) germaria from newly eclosed (A,C,E) wild type and (B,D,F) zpg^z-5352/Df(3L)Zn47 females. (A-D) Germ cells labeled by immunofluorescence with anti-Vasa. (B,D) zpg mutant ovaries contained only a few early germ cells, usually located at the tip of the germarium. (C,D) Higher magnification views of individual germaria. (E,F) Same samples as in C,D stained with anti--Spectrin to show spectrosomes and fusomes and with anti-Fasciclin III to outline somatic cells. Germ cells (arrows in C-F) with a spherical spectrosome at the stem cell position in (C,E) wild type and (D,F) zpg^z-5352/Df(3L)Zn47 germaria.

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