
Fig. 5. Germline specific expression of zpg. (A) Northern blot showing 1.6 kb zpg transcript detected in adult males and females containing germ cells but not in agametic adults. (B) Expression of zpg mRNA in wild-type adult testes assayed by in situ hybridization: left testis probed with zpg antisense RNA. Right testis probed with sense strand control. zpg transcript is in spermatogonia at the testis apical tip (arrow). (C,D) Expression of zpg mRNA in wild-type embryos assayed by in situ hybridization with zpg antisense probe. Anterior is towards the left. (C) Blastoderm stage embryo showing zpg mRNA in pole cells, (D) zpg mRNA in primordial germ cells in coalescing gonads.