Fig. 3. Paraxial mesoderm cells next to midline precursors express dlc and dld. (A,B) Dorsal views, anterior towards the top, of 8.5 hpf (A) and 9.5 hpf (B) embryos probed for dld expression. Cells at the dorsal midline (arrows) do not express dld. (C,D) Dorsal views, anterior towards the top, of 8.5 hpf (C) and 9.5 hpf (D) embryos probed for dlc expression. Similar to dld, dorsal midline cells (arrows) do not express dlc. (E,F) Transverse sections of 9.5 hpf embryos probed for dld (E) and dlc (F) expression. Paraxial mesoderm expresses dld and dlc, whereas midline (flanked by arrows) and ectoderm (asterisks) do not. (G) Dorsal view, anterior towards the top, of 9.5 hpf embryo probed for dlc (purple) and ntl (red) expression. (H) Transverse section of 9.5 hpf embryo probed for dlc (blue) and ntl (red) expression. In both G and H, dlc-expressing paraxial mesoderm borders ntl-positive midline precursors (arrows). (I) Transverse section of 9.5 hpf embryo showing her4-positive cells (red, arrows) adjacent to dlc-expressing cells (blue, arrows). Scale bar: 100 µm for A-D,G; 20 µm for E,F,H,I.
