Fig. 7. pygo encodes a nuclear protein that acts downstream of Arm nuclear import. (A-I) Confocal images of wing imaginal discs stained for Arm (red) and a NPC antigen (green). Arrows indicate nucleoplasm. Clones (indicated by the white lines) of pygo10 (A,D,G), Axin (B,E,H) and Axin, pygo10 (C,F,I) indicate that the double mutant phenotype (high levels of nuclear Arm; see arrows in H,I) is nearly identical to Axin. (J-L) Confocal image of a S2 cell transiently transfected with a GFP-Pygo chimeric gene driven by the constitutive Actin5C promoter. NPC staining is shown in red and GFP in green. Almost all the GFP signal is found in the nucleus. All images are single optical slices of <1 µm depth.
