Fig. 7. EPL cell-derived neurectoderm can be directed to neural crest and glial lineages (A-D). EBM9 explants were seeded onto cellular fibronectin-treated tissue culture plasticware in medium supplemented with 25 nM staurosporine/0.1% DMSO (A,C,D) or 0.1% DMSO alone (B). Cultures were examined after 3 (A,B) or 48 hours (C,D). (D) In situ hybridisation analysis of EBM9 explants with digoxigenin-labelled antisense probes for Sox10. (E-H) EBM9 explants were seeded onto poly-L-ornithine-treated tissue culture plasticware in medium supplemented with 10 ng/ml FGF2, 20 ng/ml EGF and 1 µg/ml laminin (E,F) followed by culture in medium supplemented with 10 ng/ml PDGF-AA (G,H). Cultures were examined after 2 (A), 4 (F) and 6 (G,H) days. (H) Immunohistochemistry of EBM9 explants with antibodies directed against glial fibrillary acidic protein (GFAP).
