Fig. 3. bcd mRNA is stabilized in pum^– mutants. RNAs used in Fig. 2 analyzed by northern blot. (A) Probed for bcd and rpA1. bcd is stabilized in pum^– mutants compared with wild type (lane numbering as in Fig. 2). Similar results were obtained for pum¹³/pum¹³, pum¹/pum¹³ and pum^Msc/pum^FC8 embryos. (B) Reprobing for hb shows the embryo populations are developing synchronously and hb^mat is also stabilized in pum^– mutants. (C) Western blot of wild type (lanes 1-9) and pum^– (1'-9') embryo proteins probed for Bicoid (time course identical to Fig. 2, and A,B). Lane C, in vitro translated unlabelled Bicoid protein. The lower panel (same gel) shows a secondary antibody crossreacting band that acts as a loading control.

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