Fig. 5. Wolffian duct in 11 dpc embryos. The mesonephric kidney is comparably developed in 
1III4-deficient (B) and in wild-type (A) embryos (arrows). (C,D) Growth of the Wolffian duct (arrowheads) can be followed in consecutive sagittal sections throughout the urogenital ridge in wild-type (C) but not in mutant (D) embryos. A representative sections of the blind-ending Wolffian duct in mutant embryos is shown in D. Confocal microscopy after immunostaining for laminin (E-G) and BrdU (red label in G) reveals continuous basement membranes structures in the controls (E) and mutants (F), which are, however, partially interrupted (arrows) in deeper layers of the section (G). Pax2 (H,I) is normally present in the Wolffian duct in the mutants (I) compared with littermate control embryos (H). Scale bars: 100 µm in A,B; 250 µm in C,D; 40 µm in E-G; 50 µm in H,I.
