Fig. 1. Targeted disruption of Tcfap2c (the gene for AP-2
) and representative genotype analysis of 7.5 d.p.c. embryos from an Tcfap2c+/– intercross. (A) Derivation of an Tcfap2c-null allele. The wild-type Tcfap2c gene (top), targeting vector (middle) and recombinant locus (bottom). Exons 6 and 7 (black boxes), and restriction enzyme sites (B, BamHI; C, ClaI; H, HindIII; P, PstI; N, NcoI) are shown along with the neomycin resistance marker (neo) and two copies of the HSV thymidine kinase gene (tk). The positions of the primer pairs used for ES colony screening by PCR amplification are shown beneath, as are the positions of the 5' and 3' probes used for Southern blot analysis (checked boxes). The position of the primers used for identifying the wild-type allele in subsequent mice are shown towards the top of the figure (Gamwt5 and Xgamma3'). (B) DNA samples were subjected to PCR analysis using a mixture of three primers (see Materials and Methods). Amplification of the wild-type allele produces a fragment of 190 bp (upper band), while amplification of the targeted allele results in a 140 bp product (lower band). Abbreviations: WT, wild type; KO, size of Tcfap2c–/– allele PCR product.
