Fig. 2. Analyses of gross morphology (A) and expression of Tcfap2c (B) in embryos from Tcfap2c+/– intercrosses. (A, parts a, d) Wild type late allantoic bud stage embryo recovered at 7.5 d.p.c., covered by parietal endoderm/Reichert’s membrane and associated trophoblast cells (part a) and with Reichert’s membrane dissected away (part d). (A, parts b,c,e,f) Tcfap2c–/– embryos recovered at 7.5 d.p.c. (b,c,e) and 10.5 d.p.c. (A, part f). (A, part b) Tcfap2c–/– mutant with Reichert’s membrane intact. Embryos are oriented with mesometrial pole to the top and are of the same magnification. (B) RNA in situ hybridization with an AP-2
antisense probe on sagittal sections of 7.5 d.p.c. embryos from Tcfap2c+/– intercrosses. Mesometrial pole is towards the top. (B, parts a-d) Darkfield images. (B, parts e,f) Brightfield images. AP-2 transcripts localize to trophoblast derivatives in wild-type conceptuses (a,c,e) but not in Tcfap2c–/– conceptuses (b,d,f). AP-2 transcripts are present in the antimesometrial decidua of both wild type (a,c,e) and mutant (b,d,f) conceptuses. Abbreviations: –/–, Tcfap2c–/–; gc, giant cell; em, embryonic region; ex, extra-embryonic region; epc, ectoplacental cone; hf, headfold; exe, extra-embryonic ectoderm; ee, embryonic ectoderm; de, deciduum.
