Fig. 3. AP-2
protein localization from 2.5-7.5 d.p.c. (A-G) Sagittal sections of 5.5-7.5 d.p.c. embryos. The mesometrial pole is positioned towards the top, and the antimesometrial pole is to the bottom. AP-2 protein is localized to wild-type extra-embryonic tissues and is present in the trophoblast giant cells (gc), extra-embryonic ectoderm (exe), ectoplacental cone (epc) and parietal endoderm (pe). No AP-2 protein was detected in 7.5 d.p.c. Tcfap2c–/– mutants (E,G). (H-M) Whole-mount analysis of morulae and blastocysts. (H,I) Fluorescent and (J,M) DAB stained immunodetection of AP-2 protein. (K,L) DNA counterstaining with Hoechst. AP-2 protein is present in all cells of the morula (H) and in all cells of both wild-type (I,J) and Tcfap2c–/– (M) blastocysts.
