Fig. 7. RNA in situ hybridization analyses of trophoblast markers in 7.5 d.p.c. embryos from Tcfap2c+/– intercrosses. (A) Detection of transcripts for Pl1 (A, parts a-d), Ada (A, parts e-h) and Mash2 (A, parts i-l) in longitudinal sections of wild-type (A, parts a,e,i) and Tcfap2c mutant (A, parts c,g,k) conceptuses. (A, parts b,d,f,h,j,l) The equivalent brightfield images. The mesometrial pole is oriented towards the top, and the antimesometrial pole is towards the bottom. (B) Whole-mount detection of transcripts for Fgfr2 (B, parts a,b), Bmp4 (B, parts c,d), Cdx2 (B, parts e-h) and Eomes (B, parts i-l) in wild type (B, parts a,c,e,i) and Tcfap2c–/– (B, parts b,d,f-h,j-l) embryos. Mesometrial pole is towards the top. Embryos are at the same magnification. Abbreviations: WT, wild type; –/–, Tcfap2c–/–; gc, giant cell; epc, ectoplacental cone; ch, chorion; exe, extra-embryonic ectoderm; al, allantoic bud; ps, primitive streak. Scale bars: 100 µm.
