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Fig. 8. Chimeras derived from ROSA26-tagged Tcfap2c–/– ES cells (A-C) and from injection of ROSA26-tagged wild-type ES cells into blastocysts from Tcfap2c+/– intercrosses (D,E). (A) Adult chimeras generated by injection of Tcfap2c–/– ES cells into wild-type blastocysts. (B,C) X-gal-stained chimeras at 10.5 d.p.c. and 12.5 d.p.c., respectively, generated by aggregation of ROSA26-tagged Tcfap2c–/– ES cells with wild-type tetraploid embryos. (D,E) X-gal-stained littermate chimeras at 10.5 d.p.c. generated by injection of wild-type ROSA26-tagged ES cells into an Tcfap2c+/– blastocyst (D) and an Tcfap2c–/– blastocyst (E). Scale bars: 600 µm in B-E.





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