Fig. 7. (A) Histogram showing percentages of GFP-labeled, A4 cells (blue bars) or of GFP-labeled A4 cells co-cultured with wild-type adult bone marrow in a Dexter-type culture (red bars) after FACS sorting for expression of Ter119, CD45, Mac3, CD11b and Gr1. (B) A multinucleated (arrowhead), and another TRAP-positive cell are shown from a culture treated with 1,25 (OH)2 vitamin D3. (C) RT-PCR for the expression of the calcitonin receptor in control cells (c) and cells treated (t) as described above. (D-G) Morphology of GFP-positive cells, stained with Giemsa, revealing predominantly blast (D,F), macrophage (E) and immature myeloid (G) morphologies.
