Fig. 6. Vagal-to-sacral heterotopic graft, E16. (A) In the colorectum, transplanted vagal NCC colonised the myenteric plexus (mp), the submucosal plexus (smp) and were also present (arrowheads) within the circular muscle layer (cm). No immunopositive cells were observed within the mucosa. (B) Using the pan-neuronal marker ANNA-1, neurons were labelled within the submucosal plexus (smp) and myenteric plexus (mp). (C,C',C'') Double labelling with QCPN and ANNA-1 revealed that all ANNA-1-positive neurons carried the QCPN+ quail nucleus, indicating that these neurons were derived from transplanted vagal NCC. Scale bars: 100 µm in A,B; 10 µm in C''.
