Fig. 6. Expression of the LENNY (lenticular cell), DR5 (auxin maximum) and J1092 (root cap) markers in wild-type and topless mutant embryos. (A) Wild-type heart stage embryo expressing the LENNY reporter in the lenticular cell and adjacent cells. (B) Wild-type mature stage embryo expressing the LENNY reporter. (C) Wild-type mature stage embryo expressing the J1092 reporter expressed in the root cap. (D) topless embryo showing a weak signal for the LENNY reporter at the apical pole. (E) topless embryo with LENNY expression at both poles. Note that the apical pole is wider and does not yet show root-like cellular organization. (F) topless embryo with LENNY expression at both poles. (G) topless embryo arrested as an elongated embryo. Notice the two foci of LENNY expression connected to each other. (H) topless embryo arrested at globular stage. LENNY reporter is expressed throughout. (I) A rare tpl-1 embryo that expresses the DR5 reporter in the apical region of the embryo. In these rare individuals, expression is typically patchy. (J) Expression of the J1092 root cap marker in a homozygous tpl-1 mutant embryo. (K) Image in J overlaid on Nomarski image of embryo. Scale bars: 25 µm in A,G,H; 65 µm in B,C; 50 µm in D-F,I-K.
