Fig. 9. Tiling in stan^E59 (fmi^E59) and seq²² mutant neurons. (A) MARCM scheme for producing stan^E59 (fmi^E59) mutant clones. ‘UAS’ indicates a UAS-mCD8::GFP insertion. For producing seq clones FRT42D was used in place of FRTG13. (B,C) stan^E59/stan^E59 (fmi^E59/fmi^E59) segmental homologs. The dendrites of these ddaC neurons stop at the lateral margin (arrow in B) where they meet the dendrites of an adjacent class IV neuron. (C,C') A high-magnification view of the region surrounding the arrowhead in B, where dendrites show normal self-avoidance and are excluded from the territory of a segmental homolog. (D) Dendritic overextension phenotype seen in a stan (fmi) mutant ddaC neuron. A white arrowhead identifies the cell body. The white arrows identify a branch extending beyond the segment border along the dorsal midline. The dorsal and ventral boundaries of the cell are delineated by white lines. (E) Dendritic overextension in the ventral neuron, vdaB. A white arrowhead identifies the cell body. Arrows follow the dorsal and ventral extensions. The remaining branches innervate their normal territory in the ventral body wall. (F,F') Dendritic field formation by seq²²/seq²² type IV neurons appears normal. As shown here, dendrites of v’ada segmental homologs (traced in red and green in F') avoid each other’s territories and form normal boundaries at the segment border (large star). In some areas near the segment border, the cell of origin of individual dendrites is difficult to determine (small star), although obvious dendrite crossing was not observed. Dorsal is up and anterior is to the left. Scale bar, 25 µm (C,C'); 50 µm (B,F,F'); 100 µm (D,E).

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