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Fig. 6. Deferred implantation leading to retarded development and poor pregnancy outcome in wild-type mice. (A) Implantation of day 4 wild-type blastocysts transferred into wild-type recipients on day 4 (n=5) or 5 (n=7) of pseudopregnancy. The numbers above the bars indicate the number of implantation sites per total number of blastocysts transferred. Implantation sites were recorded 48 hours later by the blue dye method. Implantation rate was similar between the two groups. (B) Postimplantation developments of wild-type blastocysts transferred into wild-type recipients on day 4 (n=3) or 5 (n=4) of pseudopregnancy. Implantation sites were examined 8 days later after blastocyst transfer equivalent to day 12 of pregnancy. While resorption and retarded feto-placental growth were frequent in recipients that received blastocyst transfers on day 5, vastly normal development was noted in those receiving blastocyst transfer on day 4. Normal implantation sites (IS) represent sites with normally developing embryos, while retarded IS represents resorption sites and IS with retarded embryo development. On day 12, the number of normal IS was significantly higher in day 4 recipients than in day 5 recipients (18/23 versus 10/42; 
2-test; P<0.001). (C) The pregnancy outcome of wild-type blastocysts transferred into wild-type recipients on day 4 (n=13) or 5 (n=11) of pseudopregnancy. The numbers above the bars indicate the number of pups delivered at term/total number of blastocysts transferred (2-test; *P<0.01). The number of pups born was significantly lower for mothers receiving blastocyst transfers on day 5. (D) Decidualization in wild-type mice. Mice received intraluminal oil infusion on day 4 or 5 of pseudopregnancy. Uterine weights were recorded 4 days later. Fold increases denote comparison of weights between infused and non-infused uterine horns. The numbers above the bars indicate the number of responding mice/total number of mice. No significant difference in decidualization was noted between these two groups (unpaired t-test; P>0.05).
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