Fig. 2. Properties of her7-targeted morpholinos. The efficacy and specificity of the morpholinos targeted to her7 in preventing translation (B-I) and in stabilizing target mRNAs (J-L) are shown. The structure of the 5'UTR of the her7 mRNA is shown in A, illustrating the binding sites of the morpholinos her7m1 (spanning the ATG) and her7m2 (more 5'). The uppercase C indicates a consistent sequence difference in TLF, *AB and GH (recently derived) strains from the database sequence. The relationship of the 5'UTR to the entire her7 transcript, as well as to control constructs using the gfp-coding region is shown below in block form. GFP fluorescence is shown in living embryos between 6 and 10 somite stages in B,D,F,H, and the presence of injected gfp mRNA is shown in whole mount at the same stage (C,E,G,I). (B,C) Injected with gfp mRNA; (D,E) co-injected with gfp mRNA and 7 ng/µl her7m2; (F,G) her7 5'UTR-gfp and control morpholino; and (H,I) her7 5'UTR-gfp and 7 ng/µl her7m2. The distribution of endogenous and exogenous her7 mRNA at bud stage (10 hpf) is shown in uninjected whole mounted embryos (J), after injection of her7 mRNA (K), and co-injection of her7 mRNA and 5 ng/µl her7m1 (L).
