Fig. 8. Effect of reduction of her1 and her7 function on somite morphogenesis. The appearance of the paraxial mesoderm in live embryos is shown in lateral view at 11.5 hpf (four somites; A,B) and 14 hpf (10 somites; C,D) in uninjected wild type embryos (A,C) and in wild type injected with 2.5 ng/µl of both her1m1 and her7m2 (B,D). Arrowheads indicate the position of normal somitic furrows in A,C, and delayed partial furrows in D. The position of nuclei demarcating epithelial boundaries in the anterior trunk paraxial mesoderm is shown in dorsal view of flat mounted 14 hpf (10 somite) embryos stained with 1 µM Hoechst 34222 (E,F). (E) Wild type uninjected control, (F) wild type injected with 2.5 ng/µl of both her1m1 and her7m2. Arrowheads indicate the position of the first nine intersomitic furrows in E and five abnormally spaced and oriented furrows in F, which is a montage of two focal planes. Scale bar in E: 50 µm for E,F.
