Fig. 3. pipe expression is not affected by dof and rho loss-of-function clones, but by ectopic expression of rho. (A-I) Stage 10 egg chambers (anterior towards the left and dorsal towards the top). (A) A large clone of mutant cells missing dof is marked by the absence of GFP expression (green). (B) pipe expression is visualized using a pipe-lacZ construct (red). (C) The merged pictures show that pipe expression is not affected by loss of dof activity. (D) A large clone lacking rho function is marked by the absence of GFP. (E) pipe expression is visualized using the pipe-lacZ construct. (F) The merged pictures show that pipe expression is not affected by loss of rho activity. (G) rho flip-out clones marked by GFP expression. (H) pipe expression is visualized using the pipe-lacZ construct. (I) The merged pictures show that ectopic rho expression (green) leads to cell-non-autonomous repression of pipe (red).
