Fig. 6. The effects of dec-marked Raf clones of variable sizes on the larval cuticle. Darkfield micrographs of the (A1,B1) dorsal or (C1) lateral surface of eggs focusing on the outer egg shell (chorion). The chorion derived from dec follicle cell clones appears to be more transparent. Anterior is towards the left. (A2,B2,C2) Camera lucida drawings of the pictures shown in A1,B1,C1. The dec clones are marked in pink. (A3,B3,C3) Nomarski micrographs showing the larval cuticle inside the eggs. (A4,B4,C4) Camera lucida drawings of the larval cuticles. (A5,B5,C5) Phase-contrast micrographs showing magnified views of the regions of the cuticle boxed in A3,B3,C3. (A) Egg carrying multiple dorsal dec Raf clones that contains a larva with normal cuticle. At the dorsal side facing the clones the cuticle harbours dorsal hairs (A5). (B) Egg carrying a large dorsal dec Raf clone, which comprises about 40 follicle cells and spans up to 10 follicle cells in the mediolateral dimension. The larva that developed inside this egg has a normal cuticle. At the dorsal side facing the clone, the cuticle harbours dorsal hairs (B5). (C) Egg carrying a large lateral dec Raf clone that comprises about 40 follicle cells. The larva that developed inside this egg shows cuticular defects in the head and thorax region which might be caused by a local ventralization. At the posterior pole, the presence of normally sized ventral denticle belts (vd) and extended filzkörper (fk, B5) demonstrates normal DV patterning.
