Fig. 2. Expression of Runx2 in MMCs. (A) RT-PCR analysis of the expression of MyoD (lane 2), Runx2 (lane 3) and PPAR
(lane 4) expression in undifferentiated MMCs cultured in pmGM. The abundance of Gapdh mRNA was examined as a positive control (lane 1). DNA size markers in lane M represent 200, 300 and 400 bp. (B) RT-PCR analysis of the expression of Runx2, osteocalcin (Osc) and muscle creatine kinase (MCK) in undifferentiated growing (Gr), myogenically differentiated (Myo) and osteogenically differentiated (Ost) MMCs. The abundance of Gapdh mRNA was again analyzed as a positive control. Three independent samples were analyzed for each experimental condition. (C) Immunofluorescence analysis of Runx2 expression in undifferentiated MMCs. (D) Nuclei in the same field as that shown in C were visualized by DAPI staining. (E) Immunoblot analysis of Runx2 expression in total lysates (20 µg of protein) of undifferentiated MMCs (lane 1) and C2C12 cells (lane 2). Scale bar: 100 µm.
