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Fig. 6. p53 and p21/WAF1 expression are induced in proliferating, {gamma}-irradiated whole organ cultures. Immunohistochemistry using CM5 anti-p53 (A-C) and Ab5 anti-p21/WAF1 (D-F) antisera was performed on whole organ cultures maintained for 96 hours in unsupplemented (untreated) medium (A,D), hormone-supplemented (B,E) or growth factor-supplemented (C,F) medium and 6 hours after treatment with 5 Gy of {gamma}-radiation. Hormone-supplemented medium contained estrogen (1 ng/ml) plus progesterone (1 µg/ml), while growth factor-supplemented medium contained EGF (20 ng/ml) plus TGF{alpha} (20 ng/ml). (Magnification 40x.)





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