Fig. 1. shh and emx2 expression sites define complementary domains of the hypothalamus. (A-K) Lateral views of brains of wild-type embryos with anterior towards the left showing expression of nk2.1a, shh and emx2 either in dark blue or red, as indicated (bottom right). Stages are indicated on the far right. Arrowheads in D show additional sites of emx2 expression in the posterior diencephalon and dorsal telencephalon. In J,K, the arrowheads indicate the PV hypothalamic region that expresses nk2.1a and emx2 but not shh. (L-M) Frontal serial sections through the hypothalamus, dorsal towards the top. The dorsal limit of nk2.1a and shh expression domain coincide (arrowheads). (N) Schematic view of nk2.1a, shh and emx2 expression in the 28 hpf brain. nk2.1a expression encompasses both shh and emx2 hypothalamic domains. h, hypothalamus; pd, posterior diencephalon, t, telencephalon; zli, zona limitans intrathalamica.
