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Fig. 4. Expression of otic and olfactory differentiation markers is reduced by loss of dlx3 and dlx7 function. (A,G,M) Wild-type, (B,H,N) control MO, (C,I,O) dlx7 MO, (D,J,P) dlx3MO, (E,K,Q) dlx3+7 MO and (F,L,R) b380 mutant embryos. (A-L) Expression of eya1 at 18 somites in (A-F) whole-mount and (G-L) flat-mount embryos. (M-R) Flat-mount in situ hybridization with anxV at 24 hours. (A-F) Lateral views, anterior towards the left. (G-R) Dorsal view, anterior towards the top of the panels. ot, otic vesicle; olf, olfactory placode; ap, anterior pituitary. Scale bars: in A, 100 µm in A-F; in G, 25 µm in G-L; in M, 25 µm in M-R.





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