Fig. 5. Shield transplants indicate the requirement for a notochordal source of laminin ß1. Results of transplanting: (A) gup mutant shield (labelled with Rhodamine-dextran, red) into a wild-type (WT) embryo; (B) sly mutant shield (red) into a wild-type embryo; (C) wild-type shield (Red) into a gup mutant embryo; (D,E) wild-type shield (red) into a sly mutant embryo; (F) wild-type shield into gup mutant embryo, stained with anti-laminin 1 antibody; and (G) wild-type shield (red) into sly mutant embryo, stained with anti-laminin 1 antibody. (F,G) Laminin 1 staining (black) is present around wild-type notochord tissue (arrowheads), but absent around mutant undifferentiated notochord (arrows). In G, transplanted wild-type tissue is labelled with Biotin-dextran and revealed with Fast Red.
