Fig. 3. Fate of MGE-derived tangentially migrating cells. (A-C) After 2 days in vitro, about 85% of E14 MGE-GFP cells (green, B) that migrate into the intermediate zone (IZ) express the neuronal marker MAP2 (A, red in C). The arrow in C delineates the border between the cortical plate (CP) and the IZ. (D-F) After 3 days in vitro, about 65% of E14 MGE-GFP cells (E, green in F) migrating in the IZ express the nuclear neuronal marker NeuN (D, red in F). (G-I) After 7 days in vitro, about 35% of E14 MGE-GFP cells (H, green in I) found in the cortex express the neurotransmitter GABA (G, red in I). Blue arrowheads indicate examples of double-labeled cells. Scale bars: 150 µm in A-C,G-I; 60 µm in D-F.
