Fig. 2. (A,B) Wild-type retinas labelled with CM1 antiserum. (A) 30 hours APF. (B) 43 hours APF. (C,D) GMRp35 retinas labelled with CM1 antiserum. (C) 30 hours APF. (D) 43 hours APF. (E,F) Enlargement of wild-type retina, 30 hours APF. (E) Apical. (F) Basal. Most labelled cells are apoptotic and basal. A minority also extend apically and are more normal morphologically (arrows). (G,H) Enlargement of GMRp35 retina, 30 hours APF. (G) Apical. (H) Basal. All the labelled cells are morphologically typical of the pigment cell lattice and extend from apical to basal. Note that activated caspases are excluded from the nuclei. (I) GMRp35 retina (43 hours APF) labelled with CM1 antiserum (green) and for Notch protein (magenta). (J) Eye from Elp/Elp adult. (K) Eye from Elp/Elp; GMRp35 adult. Facet number is comparable with J, but the pigmented, unfaceted region is much larger. (L) Enlargement of Elp/Elp eye disc. Weakly labelled cells are first seen around column 5. Labelling intensifies and cells progressively round up and fragment until most labelled cells have been eliminated posterior to column 15. (M) Enlargement of Elp/Elp; GMRp35 eye disc, apical plane. Weakly labelled cells are first seen around column 5. Labelling intensifies around the cell boundaries and highlights the normal cellular morphology. Labelled cells are maintained in the disc, not lost from the epithelium. Note that ommatidia (arrow) are protected. (N-Q) Eye imaginal discs labelled with CM1 antiserum. (N) Elp/Elp. Most cells in columns 7-15 are apoptotic. (O) Elp/Elp; GMRp35. Most undifferentiated cells posterior to the furrow are labelled. Occasional ommatidia and surrounding cells are unlabelled. In contrast to N, labelled cells are not lost from the back of the disc. (P) Oregon R (w). Scattered cell deaths are seen posterior to column 7. (Q) GMRp35. Cells protected from cell death label with CM1 but do not have apoptotic morphology.
