Fig. 7. pac mutants display loss of membrane-associated ß-catenin and enhanced cell proliferation in dorsal regions of midbrain and hindbrain. (A,B) Anti-ß catenin immunostaining on 2 µm confocal cross-sections through the hindbrain of wild-type (A) and pac^paR2.10 mutant sibling (B); 12-somite stage. (C-F) Anti-phospH3 immunostaining of wild-type sibling (C,E) and pac^fr7 mutant (D,F) embryos of the 12-somite stage; flat mounts of whole embryos (C,D), and representative cross-sections at the hindbrain level (E,F). Mitotic cells in the lateralmost position (indicated by arrowheads) are not part of the neural tube and are probably migrating neural crest cells. In A,B,E,F, the neural tube is outlined by dots. Arrows in F point to ectopic phospH3-positive cells in alar region of mutant.

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