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Fig. 9. ref-2 expression correlates with the pattern of Pn.p cell fusion in wild-type and mutant worms. Staged populations of the indicated mutants were stained with an anti-REF-2 antiserum. The Pn.p cell fusion pattern is indicated by white circles (unfused cells) and short horizontal lines (fused cells) next to the genotype. Below each cell is the anti-REF-2 staining pattern in the Pn.p cells starting just after the Pn.p cells are formed. The first two time points (first two rows) are before Pn.p cell fusion, the last four are after the time of Pn.p cell fusion. Black squares indicate that REF-2 was detected at that time in those cells, white squares indicate no REF-2 was detected and half filled squares indicate weak expression was sometimes detected.