Fig. 4. punc-53::GFP expression pattern. DIC (A) and fluorescence images of pAB::GFP expression in transgenic animals (B-H). (A-F) Embryos of successive stages. (A,B) 1.5-fold stage embryo showing a pioneering neurone growing in the head (B, arrow). (C,D) A view between the end of the pharynx and the mid-body of a three-fold stage embryo, showing expression in DA motoneurones pioneering the dorsal cord; C and D are two different focal planes. (E,F) Late three-fold stage embryo: (E) shows the ventral side, (F) the dorsal side, revealing expression in all DA motoneurones and some neurones in head and tail ganglia. (G-L) Adult stage; anterior is towards the left. (G,H) Adult tail, ventral (G) and mid-plane (H) views showing expression in a phasmid socket (PHso), sphincter muscle (sph) and neurones in the preanal ganglion (pag). (I) Adult head showing expression in the amphid socket (AMso), ALA and several neurones in the dorsal, ventral and retrovesicular ganglion (rvg), as well as pharyngeal neurones, including M5. nr, nerve ring. (J) Ventral view of the mid-body showing vulval sex muscles (vm1 and vm2) of an adult hermaphrodite. One of the four foot-like attachments is highlighted. Ventral (K) and dorsal (L) views of the male tail, showing specific expression in diagonal (diag) and spicule retractor (sp) muscles. Scale bars: 10 µm.

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