Fig. 2. ßPS integrin interacts with DLG. (A) Co-localization of DLG (red) and ßPS integrin (green) in the third instar NMJ. ßPS staining overlaps, but is not restricted to, the domain of DLG staining. (B) Immunoprecipitations of fly head extracts using protein A beads or beads coupled to either anti-kinesin or DLG were examined by western analysis. Note detectable ßPS is found only in the IP with DLG beads validating the DLG IP assay. (C) Anti-DLG immunoprecipitation (IP) experiments from adult heads were performed and western blots of total lysate (LYS), material on beads (IP-resuspended to same volume as lysate) and unbound proteins or flow through (FT) were probed with either anti-DLG or anti-ßPS antibodies. These results indicate that quantitative immunodepletion of DLG leads to a substantial reduction of ßPS from the lysate.

Return to article