Fig. 4. FAS2 is increased in mys mutant NMJ synapses. (A) Anti-FAS2 staining (green) and anti-DLG staining (red) at the third instar NMJ. FAS2 staining is significantly increased in both mys alleles. Arrowhead marks a type Ib bouton; arrow indicates a satellite bouton. Scale bar: 12 µm. High magnification views of FAS2 in NMJ boutons. Scale bar: 3 µm. (B) Quantification of the average intensity of FAS2 staining normalized to HRP (neuronal marker). Each bar represents the average of 80-100 boutons for each genotype. Error bars indicate s.e.m. Asterisks indicate significance. (C) Quantification of FAS2 in animals overexpressing wild type CaMKII. (***, P<0.001) (D) Sample western blots of FAS2 in the same mys mutants and the FAS2 mutants, fas2^e76 and fas2^e86. (E) Graph quantifying band intensities for western blot experiments. Staining intensities are normalized to wild type.

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