Fig. 2. Wg represses Da expression, which is required for proneural gene expression and bristle formation. (A) Micrograph of an adult fly head containing a small clone of da^5B5 cells (marked by absence of pigment). Bristles fail to form inside the clone. (B,C) Confocal images of pupal eye (B) and larval wing imaginal disc (C) containing da^5B5 clones and immunostained for Ac. Clonal boundaries were marked by the absence of ß-gal staining (not shown; indicated by white lines). Ac expression is markedly reduced inside the da clones in both tissues. (D) Wing imaginal disc containing a da^5B5 clone stained for Ac protein (orange) and sc mRNA (blue). The approximate location of the da clone is indicated by the reduction of Ac expression (white arrows). This area also contains reduced sc transcript. (E-H) Pupal eyes stained for Da protein. (E) Wild type; (F) P[sev-wg]; (G) P[GMR-Gal4], P[UAS-wg]; (H) sc^10-1 (deficiency removing the ac and sc loci). While Da expression is significantly repressed by ectopic Wg (F,G), it is only mildly affected by removal of ac and sc (H). All pupal eyes were at 3h APF.

Return to article