Fig. 2. Expression Nkx3.2, ActrIIa and Snr is dependent on BMP signaling. Ventral views of stained embryos at HH stage 11 (A,B), HH stage 7 (E,F) or HH stage 8 (G-J). (C,D) Transverse section through the embryos shown in A,B. The plane of sectioning is indicated. Embryos were placed in New culture and implanted with BMP2-expressing cells (A,E,G,I) or with Noggin-expressing cells (B,F,H,J) in the left (A,B,E,F,I,J), or right (G,H) LPM at HH stage 5. The embryos were subjected to whole-mount in situ hybridization with probes for Nkx3.2 (A,B), ActrII2a (E,F), or Snr (G-J). Red arrows indicate ectopic or loss-of expression as a result of the manipulation. Asterisk marks the implanted cell aggregate.

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