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Fig. 2. BMPs positively regulates Pitx2 expression. (A) Ectopic Pitx2 expression in the right LPM after BMP treatment. (B) A BMP-soaked bead applied to the left LPM (arrows) enhances Pitx2 expression. (C) A transverse section through the embryo shown in A at the level of ectopic Pitx2 expression. The red asterisk marks the bead (arrow) (D) A section through the same embryo at a level of normal Pitx2 expression. Note that the ectopic activation of Pitx2 by BMP also affects the neural tube. (E) A BMP-treated embryo 19 hours after the treatment, showing a right-looped heart and maintenance of ectopic right sided Pitx2 expression. . All the pictures are dorsal views.





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