Fig. 4. Car and Snail expression are positively regulated by BMP signaling. (A) A stage 7 embryo hybridized for Car. (B) The normal domain of expression of Car is expanded after ectopic BMP signaling to the left LPM. (C) Exogenous BMP signaling in the right LPM results in ectopic Car expression. (D) A Noggin-bead that has clearly inhibited the normal expression of Car, compare with the stage-matched control in A. (E) Application of Noggin at later stages only minimally downregulated Car expression (arrows). (F) Normal expression of Car in a stage-matched for comparison. (G) A control stage 8 embryo hybridized for Snail. (H) Exogenous BMP signaling in the left LPM results in ectopic activation of Snail. (I) BMP application on the right does not modify or upregulate the pattern of Snail expression. (J) Inhibition of Snail expression by Noggin, indicated by the arrows. (K) A pellet of Noggin-expressing cells applied to the right that completely abolished Snail expression.

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