Fig. 7. Effects of suppression of Cs-FoxD function with morpholino on induction of notochord cells. (A-F) In situ hybridization to examine Cs-Bra expression. (A) Normal 110-cell embryo, (B) Cs-FoxD morpholino-injected embryo and (C) an embryo injected with both Cs-FoxD morpholino and synthetic Cs-FoxD mRNA. (D) A 110-cell stage embryo developed from two-cell embryo whose right AB2 was injected with Cs-FoxD morpholino; (E) 110-cell stage embryo developed from four-cell embryo whose right B3 was injected with Cs-FoxD morpholino, and (F) 110-cell stage embryo developed from four-cell embryo whose right A3 was injected with Cs-FoxD morpholino. Black arrowheads indicate Cs-Bra expression in A-line notochord cells. Black arrows indicate Cs-Bra expression in B-line notochord cells. White arrowheads and white arrows indicate the failure of Cs-Bra expression. (G-I) Expression of a notochord-specific gene, Cs-fibrn. (G) Normal tailbud embryo; (H) Cs-FoxD morpholino-injected embryo, and (I) an embryo injected with both Cs-FoxD morpholino and synthetic Cs-FoxD mRNA. Scale bars: in A, 100 µm for B,C,D,E,F; in G, 100 µm for H,I.

Return to article