Fig. 4. (A-K) osk mRNA, STAU protein is normal in wild type and prt^gs/prt^gs mutant oocytes. OSK protein shows delocalisation or is missing from the posterior pole in the mutant oocyte and embryo. (L-N) PRT-GFP fusion protein and Osk protein colocalisation in oocyte. (A) osk mRNA is localised at the posterior pole in wild type and (B) prt^gs/prt^gs stage 10 oocytes. (C) STAU is localised at the posterior pole in wild-type and (D) prt^gs/prt^gs stage 10 oocyte. (E,G) Wild-type OSK localisation at the posterior pole of the stage 10 developing egg, (F) No OSK is detectable in the prt^gs/prt^gs egg chamber. (H,I) OSK protein is degraded from the posterior of prt^gs/prt^gs egg chambers, (J) OSK protein has posterior localisation in wild-type embryos, (K) there is no detectable OSK in prt^gs/prt^gs embryos. (L) PRT-GFP protein is subcortical in stage 10 wild type oocytes, (M) OSK protein is localised to the posterior pole and (N) in this stage they show colocalisation.

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