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Fig. 5. Western blot analysis of prtgs mutant ovaries. Western blot of ovary extracts probed with the anti-OSK antibody. Wild type, prtgs and Osk54/Df stands for an OSK protein null combination (osk54/Df(2)RPXT103). The bottom panel shows the same Western blot probed with anti {gamma}-Tubulin antibody as a loading control. The molecular weight of the three OSK-specific bands long OSK (71 kDa), phosphorylated short OSK (57 kDa) and unphosphorylated short OSK (55 kDa) are indicated.





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