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Fig. 3. Localization of Widerborst. (A-C) Widerborst (red), Flamingo (green) localization at 26 hours after puparium formation. Widerborst (B,C) is present on cell-internal spots and forms a distoproximal concentration gradient, unlike Flamingo (A,B), which is present on the proximal and distal cortex. (D-F) Nine optical sections separated by 0.25 µm were deconvolved and projected to give these images. Widerborst (D, green) is concentrated distally in the region of planar microtubules (red, F). (G-I) A single section from the projection shown in D-F. Widerborst (green) colocalizes exactly with microtubules (red). Coracle (blue) indicates cell boundaries. In all panels, distal is towards the right.





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