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Fig. 4. Widerborst polarizes by 18 hours after puparium formation. (A-C) Widerborst (A; green in B), microtubules (C; red in B) and coracle (blue) in wings from pupae aged for 5 hours at 29°C. Widerborst is localized to the proximal side of the cell. The arrow in B points distally, and anterior is upwards. (D-F) Widerborst (D; red in E) is polarized distally by 18 hours after puparium formation (pupae aged at 25°C). Flamingo (F; green in E) is only slightly polarized at this stage, compared with 24 hours (see Fig. 3A). Coracle (blue) outlines cell boundaries. The arrow in E points distally.