Fig. 6. Widerborst is required to organize planar microtubule webs. (A-C) dnWdb was expressed along the AP compartment boundary under the control of Ptc:GAL4. Pupal wings were fixed with paraformaldehyde and stained with phalloidin, to detect filamentous actin (A; red in B), and with anti-Wdb to detect dnWdb (C; blue in B). Cortical actin accumulates normally in dnWdb-expressing cells. The stripe of smaller cells with brighter actin staining correspond to the fourth wing vein. DnWdb-expressing cells should be compared with the larger intervien cells. Note that paraformaldehyde fixation does not preserve the polarized localization of Wdb. (D-F) dnWdb was expressed along the AP compartment boundary under the control of Ptc:GAL4. Pupal wings were fixed with methanol and stained with anti-Coracle (F; blue in E), anti-tubulin (D; green in E) and anti Wdb (red in E). The cortical organization of Coracle is normal, but microtubule structure is disturbed in dnWdb-expressing cells.

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