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Fig. 7. Injection of morpholino antisense oligonucleotides (MO) against zebrafish wdb 1 and wdb2 interferes with dorsoventral patterning and convergent extension movements during gastrulation. (A,B) The notochord, outlined by expression of ntl, is shortened and broadened in MO (1 ng)-injected (B) versus mock-injected (A) embryos. (C,D) The shape of the prechordal plate, marked by the expression of hgg is elongated in MO (1 ng)-injected (D) versus mock-injected (C) embryos. (E,F) the expression of gsc within the shield is expanded ventrally in embryos injected with 4 ng of wdb1 and wdb2 MOs (F) when compared with mock-injected embryos (E) at shield stage, indicating that these embryos are dorsalized.





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