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Fig. 2. Molecular cloning of mouse sperm PLC ${zeta}$ . (A) Clustal alignment of mouse sperm PLC ${zeta}$ with rat PLC ${delta}$ 1 (Accession number, P10688). Identical amino acids are shown in shaded black boxes, conservative substitutions in grey. (B) Schematic illustrating the predicted domain features of mouse PLC ${zeta}$ and mammalian PLC isoforms ß, ${gamma}$ , ${delta}$ , and ${epsilon}$ . (C) Sequence identity (blue) and similarity (red) between mammalian PLC isoforms (ß3, P51432; ${gamma}$ 2, AAH07565; ${delta}$ 1, P10688; ${epsilon}$ , AAG17145; and ${zeta}$ ). (D) Dendrogram illustrating phylogeny of Clustal aligned mammalian PLC sequences. Tree branch lengths, indicating amino acid substitutions per residue, were 0.298 for ${zeta}$ ; 0.309-0.322 for ${delta}$ 1-4; 0.397 for ${epsilon}$ ; 0.400-0.413 for ß1-4; and 0.412-0.417 for ${gamma}$ 1-2.

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