Fig. 5. In vitro fertilisation consistent with PLC-induced Ca²⁺ oscillations. Ca²⁺ changes in fura-red loaded mouse eggs that were either (A) in vitro fertilised with mouse sperm, or microinjected with cRNA encoding (B) PLC at 0.02 mg/ml; (C) PLC1 at 2 mg/ml; (D) ^PHPLC1 at 2 mg/ml (PH domain-deleted PLC1); (E) ^D210RPLC at 2 mg/ml. (A-I,B-I) Expanded traces of the longer-duration, first Ca²⁺ transient taken from A,B, respectively. (F) Autoradiograph following SDS-PAGE of [³⁵S]-labelled protein expressed in vitro from cRNA, lanes from left to right, of ^D210RPLC, PLC, ^PHPLC 1 and PLC1 corresponding to predicted protein sizes of 74, 74, 70 and 85 kDa, respectively.

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