Fig. 5. Repression of Drosophila limb development by DapANTP. Genotypes (B,E,H,K) AntpGal4; UAS-DmAntp; (C,F,I,L) AntpGal4; UAS-DapAntp. (A-C) Larval cuticle phenotypes. (B) Mild type II phenotype (see Table 1 legend), (C) Severe type V phenotype. Positions of Keilin’s organ, T1 and A1 denticle belts are indicated by arrowhead, cross and asterisk, respectively. (D-F) DLL expression (arrowheads). DapANTP eliminated DLL in T2 and T3 (asterisks in F). (G-L) DapANTP represses Dll through a DNA element that is a target for Drosophila UBX and ABDA. (G-I) Expression pattern of Dll-304 (purple). It reproduces DLL expression in the thorax (G) and is repressed by DapANTP (I, asterisks). (J-L) Expression of Dll-305 was not affected by either DmANTP (K) or DapANTP (L). In H and K, DmANTP was stained green to indicate the domain of ANTP expression that overlaps with T2 and T3. Leg primordia are indicated by arrowheads.

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